AF5087

antibody from R&D Systems

Targeting: APOH B2G1, BG

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF5087

Provider

R&D Systems

Product name

Human Apolipoprotein H/ApoH Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human Apolipoprotein H/ApoH in Western blots.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Antigen sequence

P02749


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Apolipoprotein H/ApoH by Western Blot. Western blot shows lysates of human liver tissue. PVDF membrane was probed with 1 µg/mL Goat Anti-Human Apolipoprotein H/ApoH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5087) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human ApoH (5 ng) was included. A specific band for Apolipoprotein H/ ApoH was detected at approximately 50-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Apolipoprotein H/ApoH by Simple Western^TM. Simple Western lane view shows lysates of human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Apolipoprotein H/ApoH at approximately 69 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Apolipoprotein H/ApoH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5087) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Apolipoprotein H/ApoH in Mouse Pancreas. Apolipoprotein H/ApoH was detected in perfusion fixed frozen sections of adult mouse pancreas using Goat Anti-Human Apolipoprotein H/ApoH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5087) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to pancreatic islets. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.