PA5-119665

antibody from Invitrogen Antibodies

Targeting: AMBP EDC1, HCP, HI30, IATIL, ITI, ITIL, ITILC, UTI

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-119665

Provider

Invitrogen Antibodies

Product name

alpha-1 Microglobulin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive Control: Human liver, Mouse liver, Mouse brain, Hela, HepG2, MCF-7, human liver tissue, human liver cancer tissue, human kidney tissue. Subcellular Location: Secreted.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of AMBP on different cell lysates using alpha-1 Microglobulin Polyclonal Antibody (Product # PA5-119665) at 1:1,000 dilution. Positive control: Lane 1: HepG2; Lane 2: Human liver; Lane 3: Mouse liver; Lane 4: Mouse brain.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of AMBP in HepG2 cells (green) using alpha-1 Microglobulin Polyclonal Antibody (Product # PA5-119665). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of AMBP in MCF-7 cells (green) using alpha-1 Microglobulin Polyclonal Antibody (Product # PA5-119665). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of AMBP in Hela cells (green) using alpha-1 Microglobulin Polyclonal Antibody (Product # PA5-119665). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of paraffin-embedded human liver cancer tissue using alpha-1 Microglobulin Polyclonal Antibody (Product # PA5-119665). Counter stained with hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of paraffin-embedded human liver tissue using alpha-1 Microglobulin Polyclonal Antibody (Product # PA5-119665). Counter stained with hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of paraffin-embedded human kidney tissue using alpha-1 Microglobulin Polyclonal Antibody (Product # PA5-119665). Counter stained with hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry analysis of alpha-1 Microglobulin in HepG2 cells using alpha-1 Microglobulin Polyclonal Antibody (Product # PA5-119665) at 1:100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.