RQ7218

antibody from NSJ Bioreagents

Targeting: ATP5F1B ATP5B, ATPSB

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ7218

Provider

NSJ Bioreagents

Product name

ATP5B Antibody / ATP5F1B / ATP synthase subunit beta

Antibody type

Polyclonal

Description

Antigen affinity purified

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100 µg

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the ATP5F1B antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) rat heart, 2) rat liver, 3) rat lung, 4) rat RH35, 5) mouse heart, 6) mouse liver, 7) mouse lung and 8) mouse RAW264.7 cell lysate with ATP5F1B antibody. Predicted molecular weight ~56 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) HepG2, 2) HeLa, 3) PC-3, 4) HL60, 5) T-47D, 6) Caco-2, 7) HEL and 8) K562 cell lysate with ATP5F1B antibody. Predicted molecular weight ~56 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human breast cancer tissue with ATP5F1B antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA for 20 min.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human ovarian cancer tissue with ATP5F1B antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA for 20 min.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human PC-3 cells with ATP5F1B antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human breast cancer with ATP5F1B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human endometrial adenocarcinoma tissue with ATP5F1B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human esophageal squamous carcinoma tissue with ATP5F1B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human ovarian cancer with ATP5F1B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human placental tissue with ATP5F1B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human rectal cancer tissue with ATP5F1B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE mouse heart tissue with ATP5F1B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE rat heart tissue with ATP5F1B antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human 293T cells with ATP5F1B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ATP5F1B antibody.