Explore
Validate
Learn
Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF941 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal SPARC Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human SPARC/Osteonectin in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse SPARC is observed, and less than 3% cross-reactivity with recombinant human SPARC L1 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Secreted protein acidic and rich in cysteine mediates active targeting of human serum albumin in U87MG xenograft mouse models.
In-depth proteomic analysis of tissue interstitial fluid for hepatocellular carcinoma serum biomarker discovery.
Stabilin-1 is expressed in human breast cancer and supports tumor growth in mammary adenocarcinoma mouse model.
Automated Analysis and Classification of Histological Tissue Features by Multi-Dimensional Microscopic Molecular Profiling.
Collagen signaling enhances tumor progression after anti-VEGF therapy in a murine model of pancreatic ductal adenocarcinoma.
SPARC is a key regulator of proliferation, apoptosis and invasion in human ovarian cancer.
Targeting SPARC by lentivirus-mediated RNA interference inhibits cervical cancer cell growth and metastasis.
Diverse functions of reactive cysteines facilitate unique biosynthetic processes of aggregate-prone interleukin-31.
SPARC is a VCAM-1 counter-ligand that mediates leukocyte transmigration.
Park CR, Jo JH, Song MG, Park JY, Kim YH, Youn H, Paek SH, Chung JK, Jeong JM, Lee YS, Kang KW
Theranostics 2019;9(24):7447-7457
Theranostics 2019;9(24):7447-7457
In-depth proteomic analysis of tissue interstitial fluid for hepatocellular carcinoma serum biomarker discovery.
Zhang J, Hao N, Liu W, Lu M, Sun L, Chen N, Wu M, Zhao X, Xing B, Sun W, He F
British journal of cancer 2017 Nov 21;117(11):1676-1684
British journal of cancer 2017 Nov 21;117(11):1676-1684
Stabilin-1 is expressed in human breast cancer and supports tumor growth in mammary adenocarcinoma mouse model.
Riabov V, Yin S, Song B, Avdic A, Schledzewski K, Ovsiy I, Gratchev A, Llopis Verdiell M, Sticht C, Schmuttermaier C, Schönhaber H, Weiss C, Fields AP, Simon-Keller K, Pfister F, Berlit S, Marx A, Arnold B, Goerdt S, Kzhyshkowska J
Oncotarget 2016 May 24;7(21):31097-110
Oncotarget 2016 May 24;7(21):31097-110
Automated Analysis and Classification of Histological Tissue Features by Multi-Dimensional Microscopic Molecular Profiling.
Riordan DP, Varma S, West RB, Brown PO
PloS one 2015;10(7):e0128975
PloS one 2015;10(7):e0128975
Collagen signaling enhances tumor progression after anti-VEGF therapy in a murine model of pancreatic ductal adenocarcinoma.
Aguilera KY, Rivera LB, Hur H, Carbon JG, Toombs JE, Goldstein CD, Dellinger MT, Castrillon DH, Brekken RA
Cancer research 2014 Feb 15;74(4):1032-44
Cancer research 2014 Feb 15;74(4):1032-44
SPARC is a key regulator of proliferation, apoptosis and invasion in human ovarian cancer.
Chen J, Wang M, Xi B, Xue J, He D, Zhang J, Zhao Y
PloS one 2012;7(8):e42413
PloS one 2012;7(8):e42413
Targeting SPARC by lentivirus-mediated RNA interference inhibits cervical cancer cell growth and metastasis.
Chen J, Shi D, Liu X, Fang S, Zhang J, Zhao Y
BMC cancer 2012 Oct 10;12:464
BMC cancer 2012 Oct 10;12:464
Diverse functions of reactive cysteines facilitate unique biosynthetic processes of aggregate-prone interleukin-31.
Shen M, Siu S, Byrd S, Edelmann KH, Patel N, Ketchem RR, Mehlin C, Arnett HA, Hasegawa H
Experimental cell research 2011 Apr 15;317(7):976-93
Experimental cell research 2011 Apr 15;317(7):976-93
SPARC is a VCAM-1 counter-ligand that mediates leukocyte transmigration.
Kelly KA, Allport JR, Yu AM, Sinh S, Sage EH, Gerszten RE, Weissleder R
Journal of leukocyte biology 2007 Mar;81(3):748-56
Journal of leukocyte biology 2007 Mar;81(3):748-56
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human SPARC by Simple WesternTM. Detection of Human SPARC by Simple WesternTM.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human SPARC by Western Blot. Detection of Human SPARC by Western Blot.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Human SPARC ELISA Standard Curve. Human SPARC ELISA Standard Curve.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- SPARC/Osteonectin in Human Ovary. SPARC/Osteonectin in Human Ovary.
