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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- GTX81749 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX81749, RRID:AB_11162901
- Product name
- SPARC antibody, C-term
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of SPARC Antibody (C-term) (GTX81749) in Y79 cell line lysates (35μg/lane). SPARC (arrow) was detected using the purified Pab.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of SPARC (arrow) using rabbit polyclonal SPARC Antibody (C-term) (GTX81749). 293 cell lysates (2 μg/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SPARC gene.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human skin tissue reacted with SPARC Antibody (C-term)(GTX81749), which was peroxidase-conjμgated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Flow cytometric analysis of widr cells using SPARC Antibody (C-term)(bottom histogram)(GTX81749) compared to a negative control cell (top histogram). FITC-conjμgated goat-anti-rabbit secondary antibodies were used for the analysis.
