F49838

antibody from NSJ Bioreagents

Targeting: SPARC BM-40, ON, ONT

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F49838

Provider

NSJ Bioreagents

Product name

SPARC Antibody

Antibody type

Polyclonal

Antigen

A portion of amino acids 224-251 from the human protein was used as the immunogen for this SPARC antibody.

Description

Purified antibody

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Vial size

80 µl, 400 µl

Storage

Aliquot the SPARC antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot analysis of SPARC antibody and Y79 lysate. Predicted molecular weight 35-45 kDa depending on glycosylation level

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot analysis of SPARC antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected (2) with the SPARC gene.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC analysis of FFPE human skin tissue stained with SPARC antibody

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometric analysis of WiDr cells using SPARC antibody (green) compared to a negative control (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.