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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- AF8644 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Niemann-Pick Type C2/NPC2 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Niemann-Pick Type C2/NPC2 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
P61916- Isotype
- IgG
- Vial size
- 100 uL
- Storage
- 12 months from date of receipt, -20 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after opening. 6 months, -20 °C under sterile conditions after opening.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Niemann-Pick Type C2/NPC2 by Western Blot. Western blot shows lysates of NTera-2 human testicular embryonic carcinoma cell line, human kidney (cortex) tissue, HepG2 human hepatocellular carcinoma cell line, and human skeletal muscle tissue. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human Niemann-Pick Type C2/NPC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8644) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Niemann-Pick Type C2/NPC2 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Niemann-Pick Type C2/NPC2 in SK-Hep-1 Human Cell Line. Niemann-Pick Type C2/NPC2 was detected in immersion fixed SK-Hep-1 human liver adenocarcinoma cell line using Rabbit Anti-Human Niemann-Pick Type C2/NPC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8644) at 1:150 dilution for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Niemann-Pick Type C2/NPC2 in Human Kidney. Niemann-Pick Type C2/NPC2 was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-Human Niemann-Pick Type C2/NPC2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8644) at 1:50 dilution overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in tubular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
