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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Immunoelectron microscopy [1]
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- Product number
- AB0043-500 - Provider product page
- Provider
- SICGEN
- Proper citation
- SICGEN Cat#AB0043-500, RRID:AB_2333120
- Product name
- Anti-Cathepsin D
- Antibody type
- Polyclonal
- Description
- Goat polyclonal to Caveolin-1 – caveolae marker. This scaffolding protein is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Donkey, Feline, Goat, Guinea Pig, Hamster, Horse, Porcine, Rabbit, Sheep, Simian, Other
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 1.5 mg
- Concentration
- 3 mg/ml
- Storage
- Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references LC-MS/MS Based Quantitation of ABC and SLC Transporter Proteins in Plasma Membranes of Cultured Primary Human Retinal Pigment Epithelium Cells and Immortalized ARPE19 Cell Line.
Pelkonen L, Sato K, Reinisalo M, Kidron H, Tachikawa M, Watanabe M, Uchida Y, Urtti A, Terasaki T
Molecular pharmaceutics 2017 Mar 6;14(3):605-613
Molecular pharmaceutics 2017 Mar 6;14(3):605-613
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Supportive validation
- Submitted by
- SICGEN (provider)
- Main image
- Experimental details
- Cell lysate at 100 µg and 30 µg per lane (transfected)
- Sample type
- MDCK and transfected 293FT cell line
- Primary Ab dilution
- 1/500
- Conjugate
- Unconjugated
- Secondary Ab
- Secondary Ab
- Secondary Ab dilution
- 1/10,000
Supportive validation
- Submitted by
- SICGEN (provider)
- Main image
- Experimental details
- Paraformaldehyde-fixed paraffin-embedded tissue
- Sample type
- mouse lung
- Primary Ab dilution
- 1/200
- Conjugate
- Unconjugated
- Protocol
- http://DAB
Supportive validation
- Submitted by
- SICGEN (provider)
- Main image
- Experimental details
- IEM
- Sample type
- Melanocytes
