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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [11]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- AF1029 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Cathepsin D Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse Cathepsin D in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human Cathepsin D is observed and less than 1% cross-reactivity with recombinant mouse Cathepsin E is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q3UCD9- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Histochemical characteristics of regressing vessels in the hyaloid vascular system of neonatal mice: Novel implication for vascular atrophy.
A C9ORF72/SMCR8-containing complex regulates ULK1 and plays a dual role in autophagy.
Plasma cathepsin D correlates with histological classifications of fatty liver disease in adults and responds to intervention.
RAB43 facilitates cross-presentation of cell-associated antigens by CD8α+ dendritic cells.
Renal type a intercalated cells contain albumin in organelles with aldosterone-regulated abundance.
Lysosomal protein turnover contributes to the acquisition of TGFβ-1 induced invasive properties of mammary cancer cells.
Acid sphingomyelinase modulates the autophagic process by controlling lysosomal biogenesis in Alzheimer's disease.
Pressure overload-induced cardiac remodeling and dysfunction in the absence of interleukin 6 in mice.
The cystatin M/E-cathepsin L balance is essential for tissue homeostasis in epidermis, hair follicles, and cornea.
Cellular repressor of E1A-stimulated genes is a bona fide lysosomal protein which undergoes proteolytic maturation during its biosynthesis.
Effect of immune serum and role of individual Fcgamma receptors on the intracellular distribution and survival of Salmonella enterica serovar Typhimurium in murine macrophages.
Kishimoto A, Kimura S, Nio-Kobayashi J, Takahashi-Iwanaga H, Park AM, Iwanaga T
Experimental eye research 2018 Jul;172:1-9
Experimental eye research 2018 Jul;172:1-9
A C9ORF72/SMCR8-containing complex regulates ULK1 and plays a dual role in autophagy.
Yang M, Liang C, Swaminathan K, Herrlinger S, Lai F, Shiekhattar R, Chen JF
Science advances 2016 Sep;2(9):e1601167
Science advances 2016 Sep;2(9):e1601167
Plasma cathepsin D correlates with histological classifications of fatty liver disease in adults and responds to intervention.
Walenbergh SM, Houben T, Rensen SS, Bieghs V, Hendrikx T, van Gorp PJ, Oligschlaeger Y, Jeurissen ML, Gijbels MJ, Buurman WA, Vreugdenhil AC, Greve JW, Plat J, Hofker MH, Kalhan S, Pihlajamäki J, Lindsey P, Koek GH, Shiri-Sverdlov R
Scientific reports 2016 Dec 6;6:38278
Scientific reports 2016 Dec 6;6:38278
RAB43 facilitates cross-presentation of cell-associated antigens by CD8α+ dendritic cells.
Kretzer NM, Theisen DJ, Tussiwand R, Briseño CG, Grajales-Reyes GE, Wu X, Durai V, Albring J, Bagadia P, Murphy TL, Murphy KM
The Journal of experimental medicine 2016 Dec 12;213(13):2871-2883
The Journal of experimental medicine 2016 Dec 12;213(13):2871-2883
Renal type a intercalated cells contain albumin in organelles with aldosterone-regulated abundance.
Jensen TB, Cheema MU, Szymiczek A, Damkier HH, Praetorius J
PloS one 2015;10(4):e0124902
PloS one 2015;10(4):e0124902
Lysosomal protein turnover contributes to the acquisition of TGFβ-1 induced invasive properties of mammary cancer cells.
Kern U, Wischnewski V, Biniossek ML, Schilling O, Reinheckel T
Molecular cancer 2015 Feb 15;14:39
Molecular cancer 2015 Feb 15;14:39
Acid sphingomyelinase modulates the autophagic process by controlling lysosomal biogenesis in Alzheimer's disease.
Lee JK, Jin HK, Park MH, Kim BR, Lee PH, Nakauchi H, Carter JE, He X, Schuchman EH, Bae JS
The Journal of experimental medicine 2014 Jul 28;211(8):1551-70
The Journal of experimental medicine 2014 Jul 28;211(8):1551-70
Pressure overload-induced cardiac remodeling and dysfunction in the absence of interleukin 6 in mice.
Lai NC, Gao MH, Tang E, Tang R, Guo T, Dalton ND, Deng A, Tang T
Laboratory investigation; a journal of technical methods and pathology 2012 Nov;92(11):1518-26
Laboratory investigation; a journal of technical methods and pathology 2012 Nov;92(11):1518-26
The cystatin M/E-cathepsin L balance is essential for tissue homeostasis in epidermis, hair follicles, and cornea.
Zeeuwen PL, van Vlijmen-Willems IM, Cheng T, Rodijk-Olthuis D, Hitomi K, Hara-Nishimura I, John S, Smyth N, Reinheckel T, Hendriks WJ, Schalkwijk J
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2010 Oct;24(10):3744-55
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2010 Oct;24(10):3744-55
Cellular repressor of E1A-stimulated genes is a bona fide lysosomal protein which undergoes proteolytic maturation during its biosynthesis.
Schähs P, Weidinger P, Probst OC, Svoboda B, Stadlmann J, Beug H, Waerner T, Mach L
Experimental cell research 2008 Oct 1;314(16):3036-47
Experimental cell research 2008 Oct 1;314(16):3036-47
Effect of immune serum and role of individual Fcgamma receptors on the intracellular distribution and survival of Salmonella enterica serovar Typhimurium in murine macrophages.
Uppington H, Menager N, Boross P, Wood J, Sheppard M, Verbeek S, Mastroeni P
Immunology 2006 Oct;119(2):147-58
Immunology 2006 Oct;119(2):147-58
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse Cathepsin D by Western Blot. Western blot shows lysate of mouse intestine tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Cathepsin D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1029) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for Cathepsin D at approximately 28 and 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cathepsin D in RAW 264.7 Mouse Cell Line. Cathepsin D was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Goat Anti-Mouse Cathepsin D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1029) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to lysosomes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cathepsin D in Mouse Intestine. Cathepsin D was detected in perfusion fixed frozen sections of mouse intestine using 15 µg/mL Goat Anti-Mouse Cathepsin D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1029) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter-stained with hematoxylin (blue). Specific labeling was localized to epithelial cells in intestinal villi. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
