Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB1083 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB1083, RRID:AB_1574158
- Product name
- CTSD monoclonal antibody, clone 4G2
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant CTSD.
- Isotype
- IgG
- Antibody clone number
- 4G2
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Biological and clinical significance of cathepsin D in breast cancer metastasis.
Isolation and sequencing of a cDNA clone encoding rat liver lysosomal cathepsin D and the structure of three forms of mature enzymes.
Cathepsin D in breast cancer: from molecular and cellular biology to clinical applications.
Garcia M, Platet N, Liaudet E, Laurent V, Derocq D, Brouillet JP, Rochefort H
Stem cells (Dayton, Ohio) 1996 Nov;14(6):642-50
Stem cells (Dayton, Ohio) 1996 Nov;14(6):642-50
Isolation and sequencing of a cDNA clone encoding rat liver lysosomal cathepsin D and the structure of three forms of mature enzymes.
Fujita H, Tanaka Y, Noguchi Y, Kono A, Himeno M, Kato K
Biochemical and biophysical research communications 1991 Aug 30;179(1):190-6
Biochemical and biophysical research communications 1991 Aug 30;179(1):190-6
Cathepsin D in breast cancer: from molecular and cellular biology to clinical applications.
Rochefort H, Capony F, Garcia M
Cancer cells (Cold Spring Harbor, N.Y. : 1989) 1990 Dec;2(12):383-8
Cancer cells (Cold Spring Harbor, N.Y. : 1989) 1990 Dec;2(12):383-8
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of cell lysates (each 20 ug). Lane 1 : HepG2. Lane 2 : Hep3B. Lane 3 : MDA - MB - 231. Lane 4 : MCF - 7. To probed with CTSD monoclonal antibody , clone 4G2 (1 : 1000) (Cat # MAB1083). Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and ECL detection system.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Paraffin embedded sections of human breast lobule tissue were incubated with CTSD monoclonal antibody, clone 4G2 (Cat # MAB1083 ; 1 : 50) for 2 hours at room temperature.Antigen retrieval was performed in 100 mM sodium citrate buffer and detected using Diaminobenzidine (DAB).
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
