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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [2]
- Other assay [3]
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- Product number
- STJ140018 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-CTSD antibody (275aa C-Term) (STJ140018)
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody anti-Cathepsin D (275aa C-Term) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immune Electron Microscopy research applications.
- Reactivity
- Human, Mouse, Rat, Canine, Simian
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
NA- Epitope
- NA
- Isotype
- IgG
- Antibody clone number
- NA
- Vial size
- NA
- Concentration
- NA
- Storage
- For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
- Handling
- NA
No comments: Submit comment
Supportive validation
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunofluorescence – anti-CTSD antibody at 1:100 dilution in RAW264.7 cells; cells were fixed with PFA and permeabilized with 0.05% saponin
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunofluorescence – anti-CTSD antibody at 1:100 dilution in NHI:3T3 cells; cells were fixed with methanol and permeabilized with 0.1% saponin
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
Supportive validation
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Anti-CTSD antibody at 1:500 dilution; endogenous CTSD (100 µg per lane) and transfected 293FT cell lysate (at 30 µg per lane) ; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunoperoxidase of polyclonal antibody to CTSD (1:200) on paraformaldehyde-fixed paraffin-embedded mouse lung
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunogold labeling using melanocytes and anti-CTSD antibody
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
