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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AM31775PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM31775PU-N, RRID:AB_10975413
- Product name
- anti Cathepsin D
- Antibody type
- Monoclonal
- Antigen
- CTSD antibody was raised against cTSD (AAH16320, 26 a.a. ~ 412 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3F12-1B9
- Vial size
- 50 µg
- Concentration
- 1,0 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western Blot analysis of CTSD expression in transfected 293T cell line by CTSD antibody. Lane 1: CTSD transfected lysate (Predicted MW: 44.6 KDa). Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged CTSD is approximately 3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunoprecipitation of CTSD transfected lysate using anti-CTSD monoclonal antibody and Protein A Magnetic Bead.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)
