AF469

antibody from Novus Biologicals

Targeting: VEGFD FIGF, VEGF-D

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF469

Provider

Novus Biologicals

Product name

Goat Polyclonal VEGF-D Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects mouse VEGF-D in direct ELISAs and Western blots. In direct ELISAs, less than 40% cross-reactivity with recombinant human (rh) VEGF-D is observed, and less than 1% cross-reactivity with recombinant mouse (rm) VEGF115, rmVEGF120, rhVEGF, rhVEGF-B, and rmVEGF165 is observed.

Reactivity

Mouse

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Dilated thin-walled blood and lymphatic vessels in human endometrium: a potential role for VEGF-D in progestin-induced break-through bleeding.

Donoghue JF, McGavigan CJ, Lederman FL, Cann LM, Fu L, Dimitriadis E, Girling JE, Rogers PA
PloS one 2012;7(2):e30916

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Liposomal honokiol inhibits VEGF-D-induced lymphangiogenesis and metastasis in xenograft tumor model.

Wen J, Fu AF, Chen LJ, Xie XJ, Yang GL, Chen XC, Wang YS, Li J, Chen P, Tang MH, Shao XM, Lu Y, Zhao X, Wei YQ
International journal of cancer 2009 Jun 1;124(11):2709-18

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Effects of acute exercise, exercise training, and diabetes on the expression of lymphangiogenic growth factors and lymphatic vessels in skeletal muscle.

Kivelä R, Silvennoinen M, Lehti M, Kainulainen H, Vihko V
American journal of physiology. Heart and circulatory physiology 2007 Oct;293(4):H2573-9

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VEGF-C is a trophic factor for neural progenitors in the vertebrate embryonic brain.

Le Bras B, Barallobre MJ, Homman-Ludiye J, Ny A, Wyns S, Tammela T, Haiko P, Karkkainen MJ, Yuan L, Muriel MP, Chatzopoulou E, Bréant C, Zalc B, Carmeliet P, Alitalo K, Eichmann A, Thomas JL
Nature neuroscience 2006 Mar;9(3):340-8

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

VEGF-D in Mouse Embryo. VEGF-D was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse VEGF-D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF469) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to seminiferous tubules in testis. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.