Antibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- AF286 - Provider product page
- Provider
- R&D Systems
- Product name
- Human VEGF-D Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human VEGF-D in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with mVEGF-D and less than 1% cross-reactivity with recombinant human (rh) VEGF-C is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
O43915
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Synaptonemal complex protein 3 is associated with lymphangiogenesis in non-small cell lung cancer patients with lymph node metastasis.
Dilated thin-walled blood and lymphatic vessels in human endometrium: a potential role for VEGF-D in progestin-induced break-through bleeding.
Radiogenic lymphangiogenesis in the skin.
Lymphatic reprogramming of microvascular endothelial cells by CEA-related cell adhesion molecule-1 via interaction with VEGFR-3 and Prox1.
Apoptosis of human macrophages by Flt-4 signaling: implications for atherosclerotic plaque pathology.
The expression pattern of vascular endothelial growth factor C and D in human esophageal normal mucosa, dysplasia and neoplasia.
Cyclooxygenase-2 induces EP1- and HER-2/Neu-dependent vascular endothelial growth factor-C up-regulation: a novel mechanism of lymphangiogenesis in lung adenocarcinoma.
Cyclooxygenase-2 induces EP1- and HER-2/Neu-dependent vascular endothelial growth factor-C up-regulation: a novel mechanism of lymphangiogenesis in lung adenocarcinoma.
Expression of vascular endothelial growth factor (VEGF)-D and its receptor, VEGF receptor 3, as a prognostic factor in endometrial carcinoma.
Kitano H, Chung JY, Noh KH, Lee YH, Kim TW, Lee SH, Eo SH, Cho HJ, Choi CH, Inoue S, Hanaoka J, Fukuoka J, Hewitt SM
Journal of translational medicine 2017 Jun 17;15(1):138
Journal of translational medicine 2017 Jun 17;15(1):138
Dilated thin-walled blood and lymphatic vessels in human endometrium: a potential role for VEGF-D in progestin-induced break-through bleeding.
Donoghue JF, McGavigan CJ, Lederman FL, Cann LM, Fu L, Dimitriadis E, Girling JE, Rogers PA
PloS one 2012;7(2):e30916
PloS one 2012;7(2):e30916
Radiogenic lymphangiogenesis in the skin.
Jackowski S, Janusch M, Fiedler E, Marsch WC, Ulbrich EJ, Gaisbauer G, Dunst J, Kerjaschki D, Helmbold P
The American journal of pathology 2007 Jul;171(1):338-48
The American journal of pathology 2007 Jul;171(1):338-48
Lymphatic reprogramming of microvascular endothelial cells by CEA-related cell adhesion molecule-1 via interaction with VEGFR-3 and Prox1.
Kilic N, Oliveira-Ferrer L, Neshat-Vahid S, Irmak S, Obst-Pernberg K, Wurmbach JH, Loges S, Kilic E, Weil J, Lauke H, Tilki D, Singer BB, Ergün S
Blood 2007 Dec 15;110(13):4223-33
Blood 2007 Dec 15;110(13):4223-33
Apoptosis of human macrophages by Flt-4 signaling: implications for atherosclerotic plaque pathology.
Schmeisser A, Christoph M, Augstein A, Marquetant R, Kasper M, Braun-Dullaeus RC, Strasser RH
Cardiovascular research 2006 Sep 1;71(4):774-84
Cardiovascular research 2006 Sep 1;71(4):774-84
The expression pattern of vascular endothelial growth factor C and D in human esophageal normal mucosa, dysplasia and neoplasia.
Ishikawa M, Kitayama J, Kazama S, Nagawa H
Hepato-gastroenterology 2004 Sep-Oct;51(59):1319-22
Hepato-gastroenterology 2004 Sep-Oct;51(59):1319-22
Cyclooxygenase-2 induces EP1- and HER-2/Neu-dependent vascular endothelial growth factor-C up-regulation: a novel mechanism of lymphangiogenesis in lung adenocarcinoma.
Su JL, Shih JY, Yen ML, Jeng YM, Chang CC, Hsieh CY, Wei LH, Yang PC, Kuo ML
Cancer research 2004 Jan 15;64(2):554-64
Cancer research 2004 Jan 15;64(2):554-64
Cyclooxygenase-2 induces EP1- and HER-2/Neu-dependent vascular endothelial growth factor-C up-regulation: a novel mechanism of lymphangiogenesis in lung adenocarcinoma.
Su JL, Shih JY, Yen ML, Jeng YM, Chang CC, Hsieh CY, Wei LH, Yang PC, Kuo ML
Cancer research 2004 Jan 15;64(2):554-64
Cancer research 2004 Jan 15;64(2):554-64
Expression of vascular endothelial growth factor (VEGF)-D and its receptor, VEGF receptor 3, as a prognostic factor in endometrial carcinoma.
Yokoyama Y, Charnock-Jones DS, Licence D, Yanaihara A, Hastings JM, Holland CM, Emoto M, Sakamoto A, Sakamoto T, Maruyama H, Sato S, Mizunuma H, Smith SK
Clinical cancer research : an official journal of the American Association for Cancer Research 2003 Apr;9(4):1361-9
Clinical cancer research : an official journal of the American Association for Cancer Research 2003 Apr;9(4):1361-9
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- VEGF-D in Human Prostate. VEGF-D was detected in immersion fixed frozen sections of human prostate (cross-section through a blood vessel) using 15 µg/mL Goat Anti-Human VEGF-D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF286) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by VEGF-D and Neutralization by Human VEGF-D Antibody. Recombinant Human VEGF-D (Catalog # 622-VD) stimulates proliferation in HMVEC human microvascular endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human VEGF-D (1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human VEGF-D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF286). The ND50 is typically 3-10 µg/mL.