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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Flow cytometry [2]
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- Product number
- TA502528 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA502528, RRID:AB_11126277
- Product name
- PON1 mouse monoclonal antibody, clone OTI2D4 (formerly 2D4)
- Antibody type
- Monoclonal
- Description
- PON1 mouse monoclonal antibody, clone OTI2D4 (formerly 2D4)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- PON1
- Isotype
- IgG
- Antibody clone number
- OTI2D4
- Vial size
- 100 µl
- Concentration
- 0.97 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PON1 (RC210356, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PON1.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Standard curve for ELISA analysis with PON1 recombinant protein (dilution range from 0.8ng/ml to 200ng/ml) using PON1 Capture Antibody (Cat# TA502610) at 5ug/ml and HRP conjugated PON1 Detection mAb (Cat# TA502528/TA502611) at 0.16ug/ml.
- Validation comment
- ELISA
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-PON1 mouse monoclonal antibody (TA502528) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PON1(RC210356).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PON1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502528)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PON1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502528)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-PON1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502528)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Hela cells, using anti-PON1 antibody(TA502528),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC210356 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PON1 antibody(TA502528), and then analyzed by flow cytometry.
- Validation comment
- FC
