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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- MAB4926 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse PON1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects endogenous human and mouse PON1 in Western blots. In Western blots, this antibody does not cross-react with recombinant human (rh) PON2 or rhPON3.
- Reactivity
- Human, Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
P27169- Isotype
- IgG
- Antibody clone number
- 453223
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human/Mouse PON1 by Western Blot. Western blot shows lysates of mouse liver tissue, human liver tissue and an enriched HDL/LDL fraction of human plasma obtained from ultracentrifugation. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse PON1 Monoclonal Antibody (Catalog # MAB4926) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for PON1 at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
