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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [4]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX109749 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX109749, RRID:AB_1952540
- Product name
- Vinculin antibody [N3C1], Internal
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Secretome protein signature of human pancreatic cancer stem-like cells.
Brandi J, Dalla Pozza E, Dando I, Biondani G, Robotti E, Jenkins R, Elliott V, Park K, Marengo E, Costello E, Scarpa A, Palmieri M, Cecconi D
Journal of proteomics 2016 Mar 16;136:1-12
Journal of proteomics 2016 Mar 16;136:1-12
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE GTX109749 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Vinculin antibody [N3C1], Internal detects VCL protein by Western blot analysis.A. 30 ?g PC-12 whole cell lysate/extractB. 30 ?g Rat2 whole cell lysate/extract5 % SDS-PAGEVinculin antibody [N3C1], Internal (GTX109749) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Vinculin antibody [N3C1], Internal detects Vinculin protein by western blot analysis. A. 30 £gg 293T whole cell lysate/extractB. 30 £gg A431 whole cell lysate/extractC. 30 £gg H1299 whole cell lysate/extract D. 30 £gg HeLa whole cell lysate/extractE. 30 £gg HepG2 whole cell lysate/extract7.5 % SDS-PAGEVinculin antibody [N3C1], Internal (GTX109749) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Mouse tissue extract (50 £gg) was separated by 5% SDS-PAGE, and the membrane was blotted with Vinculin antibody [N3C1], Internal (GTX109749) diluted at 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of human breast cancerncer, using Vinculin(GTX109749) antibody at 1:100 dilution.
