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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- GTX113294 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX113294, RRID:AB_10732545
- Product name
- Vinculin antibody [N1N3]
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references Differential expression of PKCα and -β in the zebrafish retina.
Substrate stiffness regulates filopodial activities in lung cancer cells.
Chemical proteomics identifies heterogeneous nuclear ribonucleoprotein (hnRNP) A1 as the molecular target of quercetin in its anti-cancer effects in PC-3 cells.
Haug MF, Berger M, Gesemann M, Neuhauss SCF
Histochemistry and cell biology 2019 Jan 2;
Histochemistry and cell biology 2019 Jan 2;
Substrate stiffness regulates filopodial activities in lung cancer cells.
Liou YR, Torng W, Kao YC, Sung KB, Lee CH, Kuo PL
PloS one 2014;9(2):e89767
PloS one 2014;9(2):e89767
Chemical proteomics identifies heterogeneous nuclear ribonucleoprotein (hnRNP) A1 as the molecular target of quercetin in its anti-cancer effects in PC-3 cells.
Ko CC, Chen YJ, Chen CT, Liu YC, Cheng FC, Hsu KC, Chow LP
The Journal of biological chemistry 2014 Aug 8;289(32):22078-89
The Journal of biological chemistry 2014 Aug 8;289(32):22078-89
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: NT2D1 7.5% SDS PAGE GTX113294 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ?g of whole cell lysate) A: Mouse brain 7.5% SDS PAGE GTX113294 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Vinculin antibody detects Vinculin protein by western blot analysis. Whole cell extracts (30 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Vinculin antibody (GTX113294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Vinculin antibody [N1N3] detects Vinculin protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Vinculin protein stained by Vinculin antibody [N1N3] (GTX113294) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human hepatoma, using Vinculin(GTX113294) antibody at 1:500 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Vinculin antibody [N1N3] detects Vinculin protein at cytoplasm in mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. Vinculin antibody [N1N3] (GTX113294) diluted at 1:500.
