Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- A30448 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Vinculin VCL Antibody
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for Vinculin/VCL detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IF. Reactive species: Human. Vinculin/VCL information: Molecular Weight: 123799 MW; Subcellular Localization: Cytoplasm, cytoskeleton . Cell junction, adherens junction . Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell junction, focal adhesion . Cytoplasmic face of adhesion plaques. Recruitment to cell-cell junctions occurs in a myosin II- dependent manner. Interaction with CTNNB1 is necessary for its localization to the cell-cell junctions (By similarity). Colocalizes with LIMD1 in the focal adhesions; Tissue Specificity: Metavinculin is muscle-specific.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100ul
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of lysates from HeLa cells, treated with forskolin 40nM 30', using Vinculin Antibody. The lane on the right is blocked with the synthesized peptide.
- Additional image