F54324

antibody from NSJ Bioreagents

Targeting: VCL

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F54324

Provider

NSJ Bioreagents

Product name

Vinculin Antibody

Antibody type

Polyclonal

Description

Antigen affinity purified

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.05 µl, 0.2 µl

Storage

Aliquot the Vinculin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human skeletal muscle, 2) human A431, 3) human HeLa, 4) mouse kidney, 5) rat PC-12 and 6) human HepG2 lysate with Vinculin antibody. Predicted molecular weight ~124 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE mouse skeletal muscle tissue with Vinculin antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of fixed and permeabilized mouse NIH 3T3 cells with Vinculin antibody; Blue=isotype control, Green= Vinculin antibody.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of fixed and permeabilized rat C2C12 cells with Vinculin antibody; Blue=isotype control, Green= Vinculin antibody.