Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- MA5-46988 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BRD9 Monoclonal Antibody (HL1282)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Store as concentrated solution.
- Antibody clone number
- HL1282
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BRD9 in K562 whole cell and nuclear extracts (30 µg) separated by 10% SDS-PAGE. Membrane was blotted with BRD9 Monoclonal antibody (Product # MA5-46988) using a dilution of 1:1,000 followed by HRP-conjugated anti-rabbit IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of BRD9 in HeLa cells. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BRD9 stained by BRD9 monoclonal antibody (Product # MA5-46988) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of BRD9 in paraffin-embedded mouse kidney. Samples were incubated in BRD9 Monoclonal antibody (Product # MA5-46988). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of BRD9 in paraffin-embedded mouse tissues. Samples were incubated in BRD9 Monoclonal antibody (Product # MA5-46988). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of BRD9 in paraffin-embedded rat tissues. Samples were incubated in BRD9 Monoclonal antibody (Product # MA5-46988). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.