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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- GTX114919 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX114919, RRID:AB_10621116
- Product name
- PCK2 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Mitochondrial phosphoenolpyruvate carboxykinase (PEPCK-M) regulates the cell metabolism of pancreatic neuroendocrine tumors (pNET) and de-sensitizes pNET to mTOR inhibitors.
Chu PY, Jiang SS, Shan YS, Hung WC, Chen MH, Lin HY, Chen YL, Tsai HJ, Chen LT
Oncotarget 2017 Nov 28;8(61):103613-103625
Oncotarget 2017 Nov 28;8(61):103613-103625
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PCK2 antibody detects PCK2 protein by western blot analysis.A. 30 ?g Neuro2A whole cell lysate/extract B. 30 ?g GL261 whole cell lysate/extract7.5% SDS-PAGEPCK2 antibody (GTX114919) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PCK2 antibody detects PCK2 protein by western blot analysis.A. 30 ?g PC-12 whole cell lysate/extract7.5% SDS-PAGEPCK2 antibody (GTX114919) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: Hep G2 (GTX27900) B: HCT116 7.5% SDS PAGE GTX114919 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PCK2 antibody (GTX114919) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PCK2 antibody detects PCK2 protein at mitochondria by immunofluorescent analysis.Sample: HepG2 cells were fixed in MeOH for 5 min.Green: PCK2 protein stained by PCK2 antibody (GTX114919) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast cancer, using PCK2(GTX114919) antibody at 1:250 dilution.
