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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Flow cytometry [1]
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- Product number
- RQ6225 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- PCK2 Antibody / PEPCK2
- Antibody type
- Monoclonal
- Description
- Affinity purified
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Mouse
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Storage
- After reconstitution, the PEPCK2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) HepG2, 2) HeLa, 3) HEK293, 4) A431, 5) monkey COS-7, 6) rat kidney, 7) mouse kidney and 8) mouse Neuro-2a cell lysate with PEPCK2 antibody. Predicted molecular weight ~71 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human HepG2 cells with PEPCK2 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human prostate cancer with PEPCK2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human breast cancer with PEPCK2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human rectal cancer with PEPCK2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human MCF7 cells with PEPCK2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PEPCK2 antibody.
