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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MA5-38591 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PCK2 Monoclonal Antibody (3D3D9)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- This antibody has been tested in indirect ELISA.
- Reactivity
- Human, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3D3D9
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PCK2 using PCK2 Monoclonal Antibody (Product # MA5-38591) in Jurkat (1), C2C12 (2), HeLa (3), HepG2 (4), COS7 (5), and HL-60 (6) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis using PCK2 Monoclonal Antibody (Product # MA5-38591) against human PCK2 (AA: 44-175) recombinant protein. (Expected MW is 40.5 kDa)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PCK2 using PCK2 Monoclonal Antibody (Product # MA5-38591) in HEK293 (1) and PCK2 (AA: 44-175)-human IgG-Fc transfected HEK293 (2) cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of PCK2 in HeLa cells using PCK2 Monoclonal Antibody (Product # MA5-38591) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Product # 35503)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of PCK2 in HeLa cells using PCK2 Monoclonal Antibody (Product # MA5-38591) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Product # 35503)
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of PCK2 in paraffin-embedded stomach cancer tissues using PCK2 Monoclonal Antibody (Product # MA5-38591) and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of PCK2 in HeLa cells using PCK2 Monoclonal Antibody (Product # MA5-38591) (green) and negative control (red).
