Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [5]
- Flow cytometry [1]
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Validation data
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- Product number
- MA5-41254 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PCK2 Recombinant Rabbit Monoclonal Antibody (JB52-39)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JB52-39
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PCK2 in human kidney tissue (1) and MCF-7 cell lysate (2) using PCK2 Recombinant Monoclonal Antibody (Product # MA5-41254) at a1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of PCK2 in HepG2 cells (green) using PCK2 Recombinant Monoclonal Antibody (Product # MA5-41254). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. The nuclear counter stain is DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of PCK2 in MCF-7 cells (green) using PCK2 Recombinant Monoclonal Antibody (Product # MA5-41254). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. The nuclear counter stain is DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of PCK2 in paraffin-embedded mouse colon tissue using PCK2 Recombinant Monoclonal Antibody (Product # MA5-41254). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of PCK2 in paraffin-embedded human kidney tissue using PCK2 Recombinant Monoclonal Antibody (Product # MA5-41254). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of PCK2 in paraffin-embedded human liver tissue using PCK2 Recombinant Monoclonal Antibody (Product # MA5-41254). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of PCK2 in paraffin-embedded rat kidney tissue using PCK2 Recombinant Monoclonal Antibody (Product # MA5-41254). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of PCK2 in paraffin-embedded human colon cancer tissue using PCK2 Recombinant Monoclonal Antibody (Product # MA5-41254). Counter stained with hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of PCK2 in MCF-7 cells. The cells were fixed, permeabilized and stained with PCK2 Recombinant Monoclonal Antibody (Product # MA5-41254) at a dilution of 1:100 (red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody. Unlabelled control (cells without incubation with primary antibody; black).