692102

antibody from BioLegend

Targeting: RBP4

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

692102

Provider

BioLegend

Proper citation

BioLegend Cat#692102, RRID:AB_2632760

Product name

Purified anti-RBP4

Antibody type

Monoclonal

Description

Retinol binding protein 4 (RBP4) is synthesized in liver where it binds vitamin A, retinol, and transports them to tissues throughout the body. In circulation, retinol-bound RBP4 is associated with transthyretin (TTR) that functions as a carrier for thyroid hormone. Binding of RBP4 to TTR prevents the loss of the smaller protein from the circulation by filtration in the glomeruli. Elevated serum RBP4 correlates with insulin resistance, obesity, and impaired glucose tolerance, indicators of metabolic syndrome such as waist-to-hip ratio (WHR), body mass index (BMI), systolic blood pressure, and decreased HDL cholesterol.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

A15103E

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Human serum (0.25 µl) or total cell lysates (15 µg protein) from HepG2 and HeLa were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL purified anti-RBP4 (clone A15103E) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin (clone 2F1-1) was used as a loading control.

Conjugate

Unconjugated