Explore
Validate
Learn
Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB3378 - Provider product page
- Provider
- R&D Systems
- Product name
- Human RBP4/Retinol-Binding Protein 4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human RBP4/Retinol-Binding Protein 4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 25% cross-reactivity with recombinant mouse RBP4 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P02753- Isotype
- IgG
- Antibody clone number
- 393005
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references A multiplex immunoassay for human adipokine profiling.
Schipper HS, de Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
Clinical chemistry 2010 Aug;56(8):1320-8
Clinical chemistry 2010 Aug;56(8):1320-8
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of RBP4/Retinol-Binding Protein 4 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepato-cellular carcinoma cell line was stained with Mouse Anti-Human RBP4/Retinol-Binding Protein 4 Monoclonal Antibody (Catalog # MAB3378, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol.
