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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- GTX113236 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX113236, RRID:AB_2037478
- Product name
- MYH9 antibody [N1-2], N-term
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Centrosome guides spatial activation of Rac to control cell polarization and directed cell migration.
Comparative proteomic analysis of mouse models of pathological and physiological cardiac hypertrophy, with selection of biomarkers of pathological hypertrophy by integrative Proteogenomics.
Arv1 promotes cell division by recruiting IQGAP1 and myosin to the cleavage furrow.
The E3 ligase Ubr3 regulates Usher syndrome and MYH9 disorder proteins in the auditory organs of Drosophila and mammals.
Mass Spectrometry-Based Proteomics Identification of Enteropathogenic Escherichia coli Pedestal Constituents.
Cheng HW, Hsiao CT, Chen YQ, Huang CM, Chan SI, Chiou A, Kuo JC
Life science alliance 2019 Feb;2(1)
Life science alliance 2019 Feb;2(1)
Comparative proteomic analysis of mouse models of pathological and physiological cardiac hypertrophy, with selection of biomarkers of pathological hypertrophy by integrative Proteogenomics.
Kwon HK, Jeong H, Hwang D, Park ZY
Biochimica et biophysica acta. Proteins and proteomics 2018 Jul 23;
Biochimica et biophysica acta. Proteins and proteomics 2018 Jul 23;
Arv1 promotes cell division by recruiting IQGAP1 and myosin to the cleavage furrow.
Sundvold H, Sundvold-Gjerstad V, Malerød-Fjeld H, Haglund K, Stenmark H, Malerød L
Cell cycle (Georgetown, Tex.) 2016;15(5):628-43
Cell cycle (Georgetown, Tex.) 2016;15(5):628-43
The E3 ligase Ubr3 regulates Usher syndrome and MYH9 disorder proteins in the auditory organs of Drosophila and mammals.
Li T, Giagtzoglou N, Eberl DF, Jaiswal SN, Cai T, Godt D, Groves AK, Bellen HJ
eLife 2016 Jun 22;5
eLife 2016 Jun 22;5
Mass Spectrometry-Based Proteomics Identification of Enteropathogenic Escherichia coli Pedestal Constituents.
Law HT, Chua M, Moon KM, Foster LJ, Guttman JA
Journal of proteome research 2015 Jun 5;14(6):2520-7
Journal of proteome research 2015 Jun 5;14(6):2520-7
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MYH9 antibody [N1-2], N-term detects MYH9 protein by western blot analysis.A. 30 ?g Rat2 whole cell lysate/extract5% SDS-PAGEMYH9 antibody [N1-2], N-term (GTX113236) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MYH9 antibody [N1-2], N-term detects MYH9 protein by western blot analysis.A. 30 ?g 293T whole cell lysate/extract B. 30 ?g A431 whole cell lysate/extract C. 30 ?g HeLa whole cell lysate/extract D. 30 ?g HepG2 whole cell lysate/extract 5% SDS-PAGEMYH9 antibody [N1-2], N-term (GTX113236) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MYH9 antibody [N1-2], N-term detects MYH9 protein by western blot analysis.A. 30 ?g NIH-3T3 whole cell lysate/extract B. 30 ?g BCL-1 whole cell lysate/extract C. 30 ?g Raw264.7 whole cell lysate/extract D. 30 ?g C2C12 whole cell lysate/extract 5% SDS-PAGEMYH9 antibody [N1-2], N-term (GTX113236) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with MYH9 antibody [N1-2], N-term (GTX113236) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MYH9(GTX113236) antibody at 1:200 dilution.
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- GeneTex (provider)
- Main image
- Experimental details
- MYH9 antibody [N1-2], N-term detects MYH9 protein at cytoskeleton by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: MYH9 stained by MYH9 antibody [N1-2], N-term (GTX113236) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
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- Main image
- Experimental details
- Immunoprecipitation of MYH9 protein from HeLa whole cell extracts using 5 £gg of MYH9 antibody [N1-2], N-term (GTX113236).Western blot analysis was performed using MYH9 antibody [N1-2], N-term (GTX113236).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MYH9 antibody [N1-2], N-term detects MYH9 protein at cytoplasm in mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. MYH9 antibody [N1-2], N-term (GTX113236) diluted at 1:400.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MYH9 antibody [N1-2], N-term detects MYH9 protein at cytoplasm in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. MYH9 antibody [N1-2], N-term (GTX113236) diluted at 1:500.
