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LearnH00004627-M03
antibody from Abnova Corporation
Targeting: MYH9
DFNA17, EPSTS, FTNS, MHA, NMHC-II-A, NMMHCA
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00004627-M03 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00004627-M03, RRID:AB_606627
- Product name
- MYH9 monoclonal antibody (M03), clone 2B3
- Antibody type
- Monoclonal
- Antigen
- MYH9 (AAH11915, 131 a.a. ~ 220 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
- Reactivity
- Human
- Host
- Mouse
- Antigen sequence
RLKQLKRQLEEAEEEAQRANASRRKLQRELEDATE
TADAMNREVSSLKNKLRRGDLPFVVPRRMARKGAG
DGSDEEVDGKADGAEAKPAE- Isotype
- IgG
- Vial size
- 100 μg
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- MYH9 monoclonal antibody (M03), clone 2B3. Western Blot analysis of MYH9 expression in HeLa.
- Protocol
- Protocol
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of MYH9 expression in transfected 293T cell line by MYH9 monoclonal antibody (M03), clone 2B3.Lane 1: MYH9 transfected lysate (Predicted MW: 10.01 KDa).Lane 2: Non-transfected lysate.
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged MYH9 is approximately 0.03ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to MYH9 on HeLa cell . [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to MYH9 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 0.5 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
