Explore
Validate
Learn
Western blot
Immunoprecipitation
Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- A303-287A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SH3BP2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The recommended shelf life for this product is 1 year from date of receipt.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human SH3BP2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SH3BP2 antibody A303-287A used for IP at 6 µg/mg lysate. SH3BP2 was also immunoprecipitated by rabbit anti-SH3BP2 antibody A303-286A, which recognizes an upstream epitope. For blotting immunoprecipitated SH3BP2, A303-286A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
