Antibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
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Validation data
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- Product number
- R1569P - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#R1569P, RRID:AB_1007312
- Product name
- anti SH3BP2 pSer427
- Antibody type
- Polyclonal
- Antigen
- This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 422-433 of Human SH3BP3 protein (SH3 Domain Binding Protein 2).
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
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- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Figure 1. Western blot analysis is shown using Affinity Purified anti-SH3BP2 pS427 antibody to detect endogenous protein present in unstimulated human whole cell lysates). The band as indicated by the arrowheads is evident in both M059 cells (panel A) and PC-3 cells (panel B). Comparison to a molecular weight marker indicates a band of ~60 kDa corresponding to human SH3BP2 protein. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using standard techniques. Personal communication Steven Pelech, Kinexus Inc.