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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Other assay [1]
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- Product number
- MA5-15424 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ETV4 Monoclonal Antibody (1A2G3)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-15424 targets ETV4 in WB applications and shows reactivity with Human samples.
- Antibody clone number
- 1A2G3
- Concentration
- Conc. Not Determined
Submitted references IP(6)-assisted CSN-COP1 competition regulates a CRL4-ETV5 proteolytic checkpoint to safeguard glucose-induced insulin secretion.
Lin H, Yan Y, Luo Y, So WY, Wei X, Zhang X, Yang X, Zhang J, Su Y, Yang X, Zhang B, Zhang K, Jiang N, Chow BKC, Han W, Wang F, Rao F
Nature communications 2021 Apr 28;12(1):2461
Nature communications 2021 Apr 28;12(1):2461
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ETV4 using ETV4 monoclonal antibody (Product # MA5-15424) in HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY ETV4 cDNA (2).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ETV4 using ETV4 monoclonal antibody (Product # MA5-15424) in K562 cell lysate (1).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 3 Activation of the CRL4 COP1 -ETV5 degradation axis underlies glucose-induced insulin secretion and is constitutively augmented in Csn2 WT/K70E (Het) mice. a Effect of MLN4924 treatment (2 muM, 2 h) on basal and glucose-stimulated insulin secretion by wild-type and Csn2 WT/K70E islets ( n = 6, *** p < 0.001, calculated by two-way ANOVA testing the effect of 2.8 or 16.8 mM glucose in the presence of MLN4924, the other p values were calculated by two-tailed Student's t test). Data are presented as mean +- SEM. b , c Mice serum insulin levels with/without MLN4924 treatment (15 mg/kg, twice weekly for 6 weeks). Insulin levels were measured at the same time of the day to minimize fluctuation. ( n = 6, ** p = 0.001, calculated by two-way repeated measures ANOVA testing genotype x time effect for Het mice). Data are presented as mean +- SEM. c Mice body weight with/without MLN4924 treatment (15 mg/kg, twice weekly for 6 weeks; n = 6, *** p < 0.001, calculated by two-way repeated measures ANOVA testing genotype x time effect for Het mice). Data are presented as mean +- SEM. d Levels of total and neddylated Cullins, COP1, and CRL4 substrates in the pancreas after 6 h fasting to normalize variation in blood glucose levels. e Ex vivo, lentiviral shRNA-based knockdown screening for CRL4 adaptors whose depletion alters islet insulin secretion. Quantified values of insulin secretion were normalize by that of Ctrl islets and presented in Log 2 scale. f Effect of adenovirus-mediated in
