Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PAB28496 - Provider product page
- Provider
- Abnova Corporation
- Product name
- SP1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against recombinant SP1
- Antigen sequence
QVSWQTLQLQNLQVQNPQAQTITLAPMQGVSLGQT
SSSNTTLTPIASAASIPAGTVTVNAAQLSSMPGLQ
TINLSALGTSGIQVHPIQGLPLAIANAPGDHGAQL
GLHGAGGDGIHDDTAGGEEGENSPDAQPQAGRRTR
REACTCP- Isotype
- IgG
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: A-431, Lane 4: Liver, Lane 5: Tonsil with SP1 polyclonal antibody at 1:250-1:500 dilution.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescent staining of human cell line U-251MG with SP1 polyclonal antibody (Cat # PAB28496) at 1-4 ug/ml shows positivity in nuclei but not nucleoli.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human placenta with SP1 polyclonal antibody (Cat # PAB28496) shows strong nuclear positivity in trophoblastic and decidual cells at 1:200-1:500 dilution.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)