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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP11451b - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AP11451b, RRID:AB_10816797
- Product name
- SP1 Antibody (C-term P692)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- SP1 Antibody (C-term P692) (Cat. #AP11451b) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the SP1 antibody detected the SP1 protein (arrow).
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of SP1 Antibody (C-term P692)(Cat#AP11451b) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
- Primary Ab dilution
- 1:10~50
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- SP1 Antibody (C-term P692) (Cat. #AP11451b)immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SP1 Antibody (C-term P692) for immunohistochemistry. Clinical relevance has not been evaluated.
- Primary Ab dilution
- 1:50~100
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- SP1 Antibody (C-term P692) (Cat. #AP11451b) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Primary Ab dilution
- 1:10~50
