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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- GTX103031 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX103031, RRID:AB_11176348
- Product name
- SP1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: THP-1 5% SDS PAGE GTX103031 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SP1 antibody detects SP1 protein by western blot analysis. Whole cell extracts (30 £gg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody (GTX103031) diluted by 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SP1 antibody detects SP1 protein at nucleus by immunofluorescent analysis.Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SP1 protein stained by SP1 antibody (GTX103031) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 6 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SP1 antibody detects SP1 protein at nucleus on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. SP1 antibody (GTX103031) diluted at 1:250.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SP1 antibody detects SP1 protein at nucleus on human gastric carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human gastric carcinoma. SP1 antibody (GTX103031) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SP1 antibody detects SP1 protein at nucleus on human gastric carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human gastric carcinoma. SP1 antibody (GTX103031) dilution: 1:500.
