Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- ARG55600 - Provider product page
- Provider
- Arigo
- Product name
- anti-SP1 antibody
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein from Human SP1.
- Description
- Purification with Protein G.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1326CT463.109.176
- Vial size
- 100 µl
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Keep the antibody in the dark and keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
- Handling
- The antibody solution should be gently mixed before use.
No comments: Submit comment
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 35 µg of HeLa, Ramos cell line (from left to right) stained with ARG55600 anti-SP1 antibody at 1:1000 dilution.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: HeLa cells stained with ARG55600 anti-SP1 antibody (green) at 1:25 dilution. Cytoplasmic actin was counterstained with Alexa Fluor? 555 conjugated with Phalloidin (red).
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Flow Cytometry: HeLa cells stained with ARG55600 anti-SP1 antibody (green) at 1:25 dilution or isotype control antibody (blue), followed by incubation with Alexa Fluor? 488 labelled secondary antibody.
