Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AP53987PU-N - Provider product page
- Provider
- OriGene
- Product name
- SP1 (C-term) rabbit polyclonal antibody, Aff - Purified
- Antibody type
- Polyclonal
- Description
- SP1 (C-term) rabbit polyclonal antibody, Aff - Purified
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- SP1
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- lot specific
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis in K562 cell line lysates (35ug/lane) using SP1 / TSFP1 antibody . (C-term). This demonstrates the SP1 antibody detected the SP1 protein (arrow).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis with?WiDr cells?using SP1 / TSFP1?antibody . (C-term),?followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in human colon carcinoma?(Formalin-fixed, Paraffin-embedded)?using SP1 / TSFP1?antibody .? (C-term), followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of SP1?/ TSFP1 antibody for IHC; Clinical relevance has not been evaluated.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram) using SP1 / TSFP1?antibody .? (C-term), followed by FITC-conjugated goat-anti-rabbit secondary antibodie. were used for the analysis.
- Validation comment
- FC
