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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PAB18222 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB18222, RRID:AB_10716670
- Product name
- SP1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of SP1.
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Down-regulation of overexpressed sp1 protein in human fibrosarcoma cell lines inhibits tumor formation.
Sp1 plays a critical role in the transcriptional activation of the human cyclin-dependent kinase inhibitor p21(WAF1/Cip1) gene by the p53 tumor suppressor protein.
Reversion of transcriptional repression of Sp1 by 5 aza-2' deoxycytidine restores TGF-beta type II receptor expression in the pancreatic cancer cell line MIA PaCa-2.
Lou Z, O'Reilly S, Liang H, Maher VM, Sleight SD, McCormick JJ
Cancer research 2005 Feb 1;65(3):1007-17
Cancer research 2005 Feb 1;65(3):1007-17
Sp1 plays a critical role in the transcriptional activation of the human cyclin-dependent kinase inhibitor p21(WAF1/Cip1) gene by the p53 tumor suppressor protein.
Koutsodontis G, Tentes I, Papakosta P, Moustakas A, Kardassis D
The Journal of biological chemistry 2001 Aug 3;276(31):29116-25
The Journal of biological chemistry 2001 Aug 3;276(31):29116-25
Reversion of transcriptional repression of Sp1 by 5 aza-2' deoxycytidine restores TGF-beta type II receptor expression in the pancreatic cancer cell line MIA PaCa-2.
Venkatasubbarao K, Ammanamanchi S, Brattain MG, Mimari D, Freeman JW
Cancer research 2001 Aug 15;61(16):6239-47
Cancer research 2001 Aug 15;61(16):6239-47
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of extracts from LoVo cells, using SP1 polyclonal antibody (Cat # PAB18222).Peptide "+" means "peptide blocking".
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of A-549 cells, using SP1 polyclonal antibody (Cat # PAB18222).Peptide "+" means "peptide blocking".
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human brain tissue using SP1 polyclonal antibody (Cat # PAB18222).Peptide "+" means "peptide blocking".
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
