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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Western blot [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- 17-601 - Provider product page
- Provider
- EMD Millipore
- Proper citation
- Millipore Cat#17-601, RRID:AB_916354
- Product name
- ChIPAb+ Sp1 - ChIP Validated Antibody and Primer Set
- Antibody type
- Polyclonal
- Antigen
- Full length human Sp1 protein
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 25 assays
- Storage
- 1 year at -20°C from date of shipment.
Submitted references Combined chromatin and expression analysis reveals specific regulatory mechanisms within cytokine genes in the macrophage early immune response.
The inducible tissue-specific expression of the human IL-3/GM-CSF locus is controlled by a complex array of developmentally regulated enhancers.
Chromatin modifications induced by the AML1-ETO fusion protein reversibly silence its genomic targets through AML1 and Sp1 binding motifs.
Identification and characterization of the human NOL7 gene promoter.
Transcription factor Sp3 is essential for post-natal survival and late tooth development.
Histone deacetylase 1 can repress transcription by binding to Sp1.
Iglesias MJ, Reilly SJ, Emanuelsson O, Sennblad B, Pirmoradian Najafabadi M, Folkersen L, Mälarstig A, Lagergren J, Eriksson P, Hamsten A, Odeberg J
PloS one 2012;7(2):e32306
PloS one 2012;7(2):e32306
The inducible tissue-specific expression of the human IL-3/GM-CSF locus is controlled by a complex array of developmentally regulated enhancers.
Baxter EW, Mirabella F, Bowers SR, James SR, Bonavita AM, Bertrand E, Strogantsev R, Hawwari A, Bert AG, Gonzalez de Arce A, West AG, Bonifer C, Cockerill PN
Journal of immunology (Baltimore, Md. : 1950) 2012 Nov 1;189(9):4459-69
Journal of immunology (Baltimore, Md. : 1950) 2012 Nov 1;189(9):4459-69
Chromatin modifications induced by the AML1-ETO fusion protein reversibly silence its genomic targets through AML1 and Sp1 binding motifs.
Maiques-Diaz A, Chou FS, Wunderlich M, Gómez-López G, Jacinto FV, Rodriguez-Perales S, Larrayoz MJ, Calasanz MJ, Mulloy JC, Cigudosa JC, Alvarez S
Leukemia 2012 Jun;26(6):1329-37
Leukemia 2012 Jun;26(6):1329-37
Identification and characterization of the human NOL7 gene promoter.
Mankame TP, Zhou G, Lingen MW
Gene 2010 May 15;456(1-2):36-44
Gene 2010 May 15;456(1-2):36-44
Transcription factor Sp3 is essential for post-natal survival and late tooth development.
Bouwman P, Göllner H, Elsässer HP, Eckhoff G, Karis A, Grosveld F, Philipsen S, Suske G
The EMBO journal 2000 Feb 15;19(4):655-61
The EMBO journal 2000 Feb 15;19(4):655-61
Histone deacetylase 1 can repress transcription by binding to Sp1.
Doetzlhofer A, Rotheneder H, Lagger G, Koranda M, Kurtev V, Brosch G, Wintersberger E, Seiser C
Molecular and cellular biology 1999 Aug;19(8):5504-11
Molecular and cellular biology 1999 Aug;19(8):5504-11
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Supportive validation
- Submitted by
- EMD Millipore (provider)
- Main image
- Experimental details
- HeLa nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Sp1 (0.2 _g/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrows indicate Sp1 (~105 and 95 kDa).
- Validation comment
- WB
Supportive validation
- Submitted by
- EMD Millipore (provider)
- Main image
- Experimental details
- ChIP Analysis: Representative lot data. Sonicated chromatin prepared from 1 x 106 Hela cells was subjected to chromatin immunoprecipitation using 4 g of either the negative control antibody, Rabbit Normal IgG or Rabbit Anti-Sp1 and the Magna ChIPª A kit (Cat.# 17-610) Rapid Protocol (2 hour IP). Successful enrichment of Sp1 associated DNA fragments was verified by qPCR using ChIP Primers DHFR, flanking the human DHFR promoter that contains a Sp1 binding site.
- Validation comment
- ChIP
