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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP53987PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP53987PU-N, RRID:AB_11151001
- Product name
- anti SP1 / TSFP1 (C-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide - KLH conjugated - corresponding to the C-terminal region (between 684-715aa) of human SP1 / TSFP1
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis in K562 cell line lysates (35ug/lane) using SP1 / TSFP1 antibody Cat.-No. AP53987PU-N (C-term). This demonstrates the SP1 antibody detected the SP1 protein (arrow).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis with WiDr cells using SP1 / TSFP1 antibody Cat.-No. AP53987PU-N (C-term), followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in human colon carcinoma (Formalin-fixed, Paraffin-embedded) using SP1 / TSFP1 antibody Cat.-No. AP53987PU-N (C-term), followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of SP1 / TSFP1 antibody for IHC; Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram) using SP1 / TSFP1 antibody Cat.-No. AP53987PU-N (C-term), followed by FITC-conjugated goat-anti-rabbit secondary antibodie. were used for the analysis.
