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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- H00006667-M02 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00006667-M02, RRID:AB_830217
- Product name
- Mouse Monoclonal SP1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. SP1 (1A5)
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references SIRT3 protects from hypoxia and staurosporine-mediated cell death by maintaining mitochondrial membrane potential and intracellular pH.
Pellegrini L, Pucci B, Villanova L, Marino ML, Marfe G, Sansone L, Vernucci E, Bellizzi D, Reali V, Fini M, Russo MA, Tafani M
Cell death and differentiation 2012 Nov;19(11):1815-25
Cell death and differentiation 2012 Nov;19(11):1815-25
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SP1 Antibody (1A5) [H00006667-M02] - SP1 monoclonal antibody (M02), clone 1A5. Analysis of SP1 expression in NIH/3T3.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: SP1 Antibody (1A5) [H00006667-M02] - Detection limit for recombinant GST tagged SP1 is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: SP1 Antibody (1A5) [H00006667-M02] - Analysis of monoclonal antibody to SP1 on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SP1 Antibody (1A5) [H00006667-M02] - Analysis of monoclonal antibody to SP1 on formalin-fixed paraffin-embedded human endometrium. Antibody concentration 1 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SP1 Antibody (1A5) [H00006667-M02] - Analysis of monoclonal antibody to SP1 on formalin-fixed paraffin-embedded human colon. Antibody concentration 1 ug/ml.
