sc-28319

antibody from Santa Cruz Biotechnology

Targeting: ZBTB16 PLZF, ZNF145

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

sc-28319

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-28319, RRID:AB_2218941

Product name

Anti-ZBTB16

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Mouse

References

Submitted references

The promyelocytic leukemia zinc-finger gene, PLZF, is frequently downregulated in malignant mesothelioma cells and contributes to cell survival.

Cheung M, Pei J, Pei Y, Jhanwar SC, Pass HI, Testa JR
Oncogene 2010 Mar 18;29(11):1633-40

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Mice deficient in the serine/threonine protein kinase VRK1 are infertile due to a progressive loss of spermatogonia.

Wiebe MS, Nichols RJ, Molitor TP, Lindgren JK, Traktman P
Biology of reproduction 2010 Jan;82(1):182-93

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

No bands detected.

Primary Ab dilution

1:500

Secondary Ab dilution

1:7000

Lane 1

Marker [kDa]: 230, 110, 82, 49.3, 32.2, 25.5, 17.6

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 74

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.

Validation comment

Staining pattern partly consistent with experimental and/or bioinformatic data.