Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
Reference
Comment
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- Product number
- sc-28319 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-28319, RRID:AB_2218941
- Product name
- Anti-ZBTB16
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
Submitted references The promyelocytic leukemia zinc-finger gene, PLZF, is frequently downregulated in malignant mesothelioma cells and contributes to cell survival.
Mice deficient in the serine/threonine protein kinase VRK1 are infertile due to a progressive loss of spermatogonia.
Cheung M, Pei J, Pei Y, Jhanwar SC, Pass HI, Testa JR
Oncogene 2010 Mar 18;29(11):1633-40
Oncogene 2010 Mar 18;29(11):1633-40
Mice deficient in the serine/threonine protein kinase VRK1 are infertile due to a progressive loss of spermatogonia.
Wiebe MS, Nichols RJ, Molitor TP, Lindgren JK, Traktman P
Biology of reproduction 2010 Jan;82(1):182-93
Biology of reproduction 2010 Jan;82(1):182-93
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- No bands detected.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:7000
- Lane 1
- Marker [kDa]: 230, 110, 82, 49.3, 32.2, 25.5, 17.6
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 74
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.
- Validation comment
- Staining pattern partly consistent with experimental and/or bioinformatic data.