PAB28457

antibody from Abnova Corporation

Targeting: ELK3 ERP, NET, SAP2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28457

Provider

Abnova Corporation

Product name

ELK3 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant ELK3.

Antigen sequence

KNIIKKVIGQKFVYKFVSFPEILKMDPHAVEISRE
SLLLQDSDCKASPEGREAHKHGLAALRSTSRNEYI
HSGLYSSFTINSLQNPPDAFKAIKTEKLEEPPEDS
PPVEEVRTVIRFVTNKTDKHVTR


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with ELK3 polyclonal antibody (Cat # PAB28457) at 1:100-1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of A-431 with ELK3 polyclonal antibody (Cat # PAB28457) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human appendix with ELK3 polyclonal antibody (Cat # PAB28457) shows moderate nuclear positivity in lymphoid tissue at 1:500-1:1000 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)