AP0350

antibody from Bioworld Technology, Inc

Targeting: TERT EST2, hEST2, TCS1, TP2, TRT

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AP0350

Provider

Bioworld Technology, Inc

Proper citation

Bioworld Technology Cat#AP0350, RRID:AB_2797500

Product name

TERT (phospho S824) polyclonal antibody

Antibody type

Polyclonal

Antigen

Synthetic phosphopeptide derived from human TERT around the phosphorylation site of Serine 824.

Description

Telomerase is an RNA-dependent DNA polymerase that catalyzes the addition of telomeric repeat sequences to chromosome ends. In most human somatic cells, telomerase activity is undetectable, and telomeres shorten with successive cell divisions. However, telomerase activity is detectable in immortal cells and in many human tumors. Two candidate mammalian telomerase proteins have been cloned. Human TP1 (for telomerase-associated protein 1), also designated TLP1 in rat (for telomerase protein component 1), is homologous to the Tetrahymena p80 telomerase protein and has been shown to interact with mammalian telomerase RNA. Human TERT (for telomerase reverse transcriptase), also designated hEST2 (for ever shorter telomeres), is homologous to the p123 telomerase protein from Euplotes and to the yeast Est2 protein. Expression of TERT mRNA has been shown to correlate with telomerase activity in various cell lines.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100ul

Concentration

1 mg/ml

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Bioworld Technology, Inc (provider)

Main image

Experimental details

Western blot (WB) analysis of p-TERT(S824) pAb at 1:500 dilutionLane1:Hela cell lysate treated with TNFa(20ng/ml,15mins)Lane2:Raw264.7 cell lysate treated with TNFa(20ng/ml,15mins)Lane3:PC12 cell lysate treated with TNFa(20ng/ml,15mins)