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ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Flow cytometry [1]
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- Product number
- ARG54933 - Provider product page
- Provider
- Arigo
- Product name
- anti-TERT antibody
- Antibody type
- Polyclonal
- Antigen
- KLH-conjugated synthetic peptide around aa. 627-656 (Center) of Human TERT.
- Description
- This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µl
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to 6 months. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 30 µg of 1) HeLa and 2) Jurkat cell line lysates stained with ARG54933 anti-TERT antibody at 1:500 dilution.Data provided by customer.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: HeLa cell stained with ARG54933 anti-TERT antibody (green) at 1:25 dilution, 1 h at 37°C. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ARG54933 anti-TERT antibody. Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TERT immunoreactivity is localized to Nucleus significantly and Cytoplasm weakly.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: SY5Y cells stained with ARG54933 anti-TERT antibody (green) at 1:200 dilution, 2 h at room temperature. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with ARG54933 anti-TERT antibody. Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min). TERT immunoreactivity is localized to the nucleus of SY5Y cells.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Flow Cytometry: HeLa cells stained with ARG54933 anti-TERT antibody (right histogram) or without primary antibody control (left histogram), followed by incubation with FITC labelled secondary antibody.
