Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- F43526 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- TERT Antibody
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acids 627-656 from the human protein was used as the immunogen for this TERT antibody.
- Description
- Purified antibody
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the TERT antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of TERT antibody and Jurkat lysate. Predicted molecular weight: 89-126 kDa (four isoforms can be visualized).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) HeLa, 2) HT-29, 3) Jurkat, 4) MOLT4, 5) PC-3, 6) 293 and 7) COLO205 cell lysate with TERT antibody. Predicted molecular weight: 89-126 kDa (four isoforms may be visualized).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Fluorescent confocal image of HeLa cell stained with TERT antibody at 1:25. Immunoreactivity is localized to the nucleus strongly and cytoplasm weakly.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- TERT antibody flow cytometric analysis of HeLa cells (green) compared to a negative control (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.