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Learnsc-48811
antibody from Santa Cruz Biotechnology
Targeting: ZNF148
BERF-1, BFCOL1, HT-BETA, pHZ-52, ZBP-89, ZFP148
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- sc-48811 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-48811, RRID:AB_794260
- Product name
- Anti-ZNF148
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references Mice over-expressing the myocardial creatine transporter develop progressive heart failure and show decreased glycolytic capacity.
Phillips D, Ten Hove M, Schneider JE, Wu CO, Sebag-Montefiore L, Aponte AM, Lygate CA, Wallis J, Clarke K, Watkins H, Balaban RS, Neubauer S
Journal of molecular and cellular cardiology 2010 Apr;48(4):582-90
Journal of molecular and cellular cardiology 2010 Apr;48(4):582-90
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Band of predicted size in kDa (+/-20%) with additional bands present.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:3000
- Lane 1
- Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 53
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human duodenum shows strong nuclear positivity in glandular cells.
- Validation comment
- Two independent antibodies targeting one protein yielding similar staining patterns. Staining pattern consistent with experimental and/or bioinformatic data.
