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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [2]
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- Product number
- MA5-17079 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FOXP1 Monoclonal Antibody (6E4C4)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-17079 targets FOXP1 in Flow cytometry, pep-ELISA, and WB applications and shows reactivity with Human samples. The MA5-17079 immunogen is purified recombinant fragment of human FOXP1 (amino acids: 481-677) expressed in E. Coli. MA5-17079 detects FOXP1 which has a predicted molecular weight of approximately 75.3kDa.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 6E4C4
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FOXP1 was performed by loading 20µg of the indicated whole cell lysates and 5µl of PageRuler Plus Prestained Protein Ladder (Product # 26619) onto a 4-20% Tris-Glycine polyacrylamide gel (Product # WT4202BX10). Proteins were transferred to a nitrocellulose membrane using the G2 Blotter (Product # 62288), and blocked with 5% Milk in TBST for 1 hour at room temperature. FoxP1 was detected at ~75kDa using a FOXP1 mouse monoclonal antibody (Product # MA5-17079) at a dilution of 1:1000 in blocking buffer for one hour at room temperature on a rocking platform, followed by a Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177) at a dilution of 1:5000 for at least one hour at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34078).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Jurkat cells using FOXP1 monoclonal antibody (Product # MA5-17079) (green) and negative control (red).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of FOXP1 was done on Jurkat cells. Cells were fixed, permeabilized and stained with a FOXP1 mouse monoclonal antibody (Product # MA5-17079, blue histogram) at a dilution of 1:100. After incubation of the primary antibody on ice for an hour, the cells were stained with a Goat anti-Mouse IgG (H+L) Secondary Antibody, DyLight 680 conjugate (Product # 35519) at a dilution of 1:50 for at least 30 minutes on ice. A representative 10,000 cells were acquired for each sample. The black histogram represents unstained control cells and the red histogram represents no-primary-antibody control.
