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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Western blot [1]
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- Product number
- AF4534 - Provider product page
- Provider
- R&D Systems
- Product name
- Human FoxP1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human FoxP1 in Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q9H334- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references The Onecut Transcription Factors Regulate Differentiation and Distribution of Dorsal Interneurons during Spinal Cord Development.
The role of Sema3-Npn-1 signaling during diaphragm innervation and muscle development.
Fasciculation and guidance of spinal motor axons in the absence of FGFR2 signaling.
Npn-1 contributes to axon-axon interactions that differentially control sensory and motor innervation of the limb.
Dynamic expression of the Onecut transcription factors HNF-6, OC-2 and OC-3 during spinal motor neuron development.
Kabayiza KU, Masgutova G, Harris A, Rucchin V, Jacob B, Clotman F
Frontiers in molecular neuroscience 2017;10:157
Frontiers in molecular neuroscience 2017;10:157
The role of Sema3-Npn-1 signaling during diaphragm innervation and muscle development.
Saller MM, Huettl RE, Hanuschick P, Amend AL, Alberton P, Aszodi A, Huber AB
Journal of cell science 2016 Sep 1;129(17):3295-308
Journal of cell science 2016 Sep 1;129(17):3295-308
Fasciculation and guidance of spinal motor axons in the absence of FGFR2 signaling.
Huettl RE, Haehl T, Huber AB
PloS one 2012;7(7):e41095
PloS one 2012;7(7):e41095
Npn-1 contributes to axon-axon interactions that differentially control sensory and motor innervation of the limb.
Huettl RE, Soellner H, Bianchi E, Novitch BG, Huber AB
PLoS biology 2011 Feb;9(2):e1001020
PLoS biology 2011 Feb;9(2):e1001020
Dynamic expression of the Onecut transcription factors HNF-6, OC-2 and OC-3 during spinal motor neuron development.
Francius C, Clotman F
Neuroscience 2010 Jan 13;165(1):116-29
Neuroscience 2010 Jan 13;165(1):116-29
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human FoxP1 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. Gels were loaded with 30 μg of whole cell lysate (WCL), 20 μg of cytoplasmic (Cyto), and 10 μg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Goat Anti-Human FoxP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4534) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for FoxP1 was detected at approximately 82 - 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
