- GTX103322 - GeneTex NFE2L2 antibody

Submitted references Bone marrow-derived mesenchymal stem cells ameliorate liver injury in a rat model of sepsis by activating Nrf2 signaling.

Selim SA, El-Baset SAA, Kattaia AAA, Askar EM, Elkader EA
Histochemistry and cell biology 2019 Mar;151(3):249-262

MicroRNA-let-7c suppresses hepatitis C virus replication by targeting Bach1 for induction of haem oxygenase-1 expression.

Chen WC, Wei CK, Lee JC
Journal of viral hepatitis 2019 Jan 31;

Autophagy Ablation in Adipocytes Induces Insulin Resistance and Reveals Roles for Lipid Peroxide and Nrf2 Signaling in Adipose-Liver Crosstalk.

Cai J, Pires KM, Ferhat M, Chaurasia B, Buffolo MA, Smalling R, Sargsyan A, Atkinson DL, Summers SA, Graham TE, Boudina S
Cell reports 2018 Nov 13;25(7):1708-1717.e5

Decrease in paracellular permeability and chemosensitivity to doxorubicin by claudin-1 in spheroid culture models of human lung adenocarcinoma A549 cells.

Akizuki R, Maruhashi R, Eguchi H, Kitabatake K, Tsukimoto M, Furuta T, Matsunaga T, Endo S, Ikari A
Biochimica et biophysica acta. Molecular cell research 2018 May;1865(5):769-780

Elevation of sensitivity to anticancer agents of human lung adenocarcinoma A549 cells by knockdown of claudin-2 expression in monolayer and spheroid culture models.

Maruhashi R, Akizuki R, Sato T, Matsunaga T, Endo S, Yamaguchi M, Yamazaki Y, Sakai H, Ikari A
Biochimica et biophysica acta. Molecular cell research 2018 Mar;1865(3):470-479

Dietary Selenomethionine Administration and Its Effects on the American Alligator (Alligator mississippiensis): Oxidative Status and Corticosterone Levels.

Finger JW Jr, Hamilton MT, Kelley MD, Zhang Y, Kavazis AN, Glenn TC, Tuberville TD
Archives of environmental contamination and toxicology 2018 Jul;75(1):37-44

Glucose starvation induces LKB1-AMPK-mediated MMP-9 expression in cancer cells.

Endo H, Owada S, Inagaki Y, Shida Y, Tatemichi M
Scientific reports 2018 Jul 4;8(1):10122

A mitohormetic response to pro-oxidant exposure in the house mouse.

Zhang Y, Humes F, Almond G, Kavazis AN, Hood WR
American journal of physiology. Regulatory, integrative and comparative physiology 2018 Jan 1;314(1):R122-R134

Zerumbone protects human skin keratinocytes against UVA-irradiated damages through Nrf2 induction.

Yang HL, Lee CL, Korivi M, Liao JW, Rajendran P, Wu JJ, Hseu YC
Biochemical pharmacology 2018 Feb;148:130-146

Antiviral effect of 7-(4-benzimidazole-butoxy)-coumarin on rhabdoviral clearance via Nrf2 activation regulated by PKCα/β phosphorylation.

Liu L, Shen YF, Hu Y, Lu JF
Fish & shellfish immunology 2018 Dec;83:386-396

A new copper ionophore DPMQ protects cells against ultraviolet B irradiation by inhibiting the TRPV1 channel.

Huang KF, Ma KH, Hung YC, Lo LC, Lin KC, Liu PS, Hu MK, Chueh SH
Journal of cellular physiology 2018 Dec;233(12):9594-9610

The Beneficial Effects of Allicin in Chronic Kidney Disease Are Comparable to Losartan.

García Trejo EMÁ, Arellano Buendía AS, Sánchez Reyes O, García Arroyo FE, Arguello García R, Loredo Mendoza ML, Tapia E, Sánchez Lozada LG, Osorio Alonso H
International journal of molecular sciences 2017 Sep 16;18(9)

A clinical drug library screen identifies clobetasol propionate as an NRF2 inhibitor with potential therapeutic efficacy in KEAP1 mutant lung cancer.

Choi EJ, Jung BJ, Lee SH, Yoo HS, Shin EA, Ko HJ, Chang S, Kim SY, Jeon SM
Oncogene 2017 Sep 14;36(37):5285-5295

Celastrol inhibits hepatitis C virus replication by upregulating heme oxygenase-1 via the JNK MAPK/Nrf2 pathway in human hepatoma cells.

Tseng CK, Hsu SP, Lin CK, Wu YH, Lee JC, Young KC
Antiviral research 2017 Oct;146:191-200

Fisetin Regulates Nrf2 Expression and the Inflammation-Related Signaling Pathway to Prevent UVB-Induced Skin Damage in Hairless Mice.

Wu PY, Lyu JL, Liu YJ, Chien TY, Hsu HC, Wen KC, Chiang HM
International journal of molecular sciences 2017 Oct 10;18(10)

Molecular mechanisms of the effects of the ethanolic extract of Muntingia calabura Linn. fruit on lipopolysaccharide-induced pro-inflammatory mediators in macrophages.

Lin JT, Chang YY, Chen YC, Shen BY, Yang DJ
Food & function 2017 Mar 22;8(3):1245-1253

No Effect of Lifelong Methylmercury Exposure on Oxidative Status in Zebra Finches (Taeniopygia guttata): A Demonstration of Methylmercury-Induced Selection?

Finger JW Jr, Botero J, Zhang Y, Still SE, Hoffman AJ, Kavazis AN, Cristol DA, Wada H
Bulletin of environmental contamination and toxicology 2017 Dec;99(6):668-672

Evaluation on antiviral activity of coumarin derivatives against spring viraemia of carp virus in epithelioma papulosum cyprini cells.

Liu L, Hu Y, Shen YF, Wang GX, Zhu B
Antiviral research 2017 Aug;144:173-185

Oral administration of methysticin improves cognitive deficits in a mouse model of Alzheimer's disease.

Fragoulis A, Siegl S, Fendt M, Jansen S, Soppa U, Brandenburg LO, Pufe T, Weis J, Wruck CJ
Redox biology 2017 Aug;12:843-853

Protective Effect of Caffeic Acid Derivatives on tert-Butyl Hydroperoxide-Induced Oxidative Hepato-Toxicity and Mitochondrial Dysfunction in HepG2 Cells.

Tsai TH, Yu CH, Chang YP, Lin YT, Huang CJ, Kuo YH, Tsai PJ
Molecules (Basel, Switzerland) 2017 Apr 28;22(5)

Sulforaphane Suppresses Hepatitis C Virus Replication by Up-Regulating Heme Oxygenase-1 Expression through PI3K/Nrf2 Pathway.

Yu JS, Chen WC, Tseng CK, Lin CK, Hsu YC, Chen YH, Lee JC
PloS one 2016;11(3):e0152236

Mycophenolate mofetil and curcumin provide comparable therapeutic benefit in experimental chronic kidney disease: role of Nrf2-Keap1 and renal dopamine pathways.

Tapia E, García-Arroyo F, Silverio O, Rodríguez-Alcocer AN, Jiménez-Flores AB, Cristobal M, Arellano AS, Soto V, Osorio-Alonso H, Molina-Jijón E, Pedraza-Chaverri J, Sanchez-Lozada LG
Free radical research 2016 Jul;50(7):781-92

Activation of the astrocytic Nrf2/ARE system ameliorates the formation of demyelinating lesions in a multiple sclerosis animal model.

Draheim T, Liessem A, Scheld M, Wilms F, Weißflog M, Denecke B, Kensler TW, Zendedel A, Beyer C, Kipp M, Wruck CJ, Fragoulis A, Clarner T
Glia 2016 Dec;64(12):2219-2230

Tolerance to ischemia reperfusion injury in a congenital heart disease model.

Asada D, Itoi T, Nakamura A, Hamaoka K
Pediatrics international : official journal of the Japan Pediatric Society 2016 Dec;58(12):1266-1273

Impaired Nrf2 regulation of mitochondrial biogenesis in rostral ventrolateral medulla on hypertension induced by systemic inflammation.

Wu KLH, Wu CW, Chao YM, Hung CY, Chan JYH
Free radical biology & medicine 2016 Aug;97:58-74

Celastrol prevents circulatory failure via induction of heme oxygenase-1 and heat shock protein 70 in endotoxemic rats.

Wang YL, Lam KK, Cheng PY, Lee YM
Journal of ethnopharmacology 2015 Mar 13;162:168-75

Neuroprotection by diarylpropionitrile in mice with spinal cord injury.

Suwanna N, Thangnipon W, Kumar S, de Vellis J
EXCLI journal 2014;13:1097-103

Andrographolide exerts anti-hepatitis C virus activity by up-regulating haeme oxygenase-1 via the p38 MAPK/Nrf2 pathway in human hepatoma cells.

Lee JC, Tseng CK, Young KC, Sun HY, Wang SW, Chen WC, Lin CK, Wu YH
British journal of pharmacology 2014 Jan;171(1):237-52

The suppression of thoc1 in cancer cell apoptosis mediated by activated macrophages is nitric oxide-dependent.

Lin YS, Lin CH, Huang LD, Chao T, Kuo CD, Hung LC, Wong FH, Lin CC, Fu SL
Biochemical pharmacology 2013 Jul 15;86(2):242-52

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Supportive validation

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Main image
Experimental details: Sample (20 ug) A: Hela whole cell lysateB: Hela cytoplasm C: Hela nucleus 7.5% SDS PAGE GTX103322 diluted at 1:1000
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (30 ug of whole cell lysate) A: NIH-3T3 7.5% SDS PAGE GTX103322 diluted at 1:2000
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: NRF2 antibody detects NRF2 protein by western blot analysis. Non-transfected (-) and NRF2-transfected (+, including 3xFlag-tag) 293T whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody (GTX103322) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: NRF2 antibody detects NRF2 protein by western blot analysis. Un-treated (-) and treated (+, 30uM tBHQ treatment for 4hr) MDA-MB-231 nuclear extract whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody (GTX103322) diluted by 1:500.
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: NRF2 antibody detects NRF2 protein by western blot analysis. Un-treated (-) and treated (+, 30?M tBHQ treatment for 4h) MDA-MB-231 NE whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NRF2 antibody (GTX103322) diluted by 1:500.
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: NRF2 antibody [N2C2], Internal detects NRF2 protein by western blot analysis.A. 30 ?g 293T whole cell extractB. 30 ?g whole cell extract of 3xFlag-human NFE2L2-transfected 293T cells7.5 % SDS-PAGENRF2 antibody [N2C2], Internal (GTX103322) dilution: 1:1000
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: NRF2 antibody detects NRF2 protein by western blot analysis. Un-treated (-) and treated (+, 30uM tBHQ treatment for 4hr) MDA-MB-231 nuclear extract whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody (GTX103322) diluted by 1:500.
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Whole cell extract (30 ?g) was separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Untreated (¡V) and treated (+) MDA-MB-231 nuclear extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NRF2 antibody (GTX103322) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Untreated (¡V) and treated (+) MDA-MB-231 nuclear extracts (30 £gg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:1000.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: NRF2 antibody detects NRF2 protein by Western blot analysis. Un-treated (-) and treated (+, 30 £gM tBHQ treatment for 4 hr) MDA-MB-231 nuclear extract (30 £gg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NRF2 antibody (GTX103322) diluted at 1:500.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: NRF2 antibody [N2C2], Internal detects NRF2 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NRF2 protein stained by NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500.Red: Phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar = 10 £gm.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: NRF2 antibody [N2C2], Internal detects NRF2 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: NIH/3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: NRF2 protein stained by NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunoprecipitation of NRF2 protein from HepG2 whole cell extracts using 5 ?g of NRF2 antibody [N2C2], Internal (GTX103322).Western blot analysis was performed using NRF2 antibody [N2C2], Internal (GTX103322).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Validation comment: IP

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunoprecipitation of NRF2 protein from HepG2 whole cell extracts using 5 £gg of NRF2 antibody [N2C2], Internal (GTX103322).Western blot analysis was performed using NRF2 antibody [N2C2], Internal (GTX103322).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using NRF2(GTX103322) antibody at 1:500 dilution.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: NRF2 antibody immunoprecipitates NRF2 protein-DNA in ChIP experiments. ChIP Sample: HeLa whole cell lysate/extract A. 5 ?g preimmune rabbit IgG B. 5 ?g of NRF2 antibody (GTX103322) The precipitated DNA was detected by PCR with primer set targeting to GCLC gene locus.
Validation comment: ChIP

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: ChIP was performed with HepG2 chromatin extract and 5 £gg of either normal rabbit IgG or anti-NRF2 antibody. The precipitated DNA was detected by PCR with primer set targeting to GCLC gene locus.

GTX103322

Antibody data